Identification of peptides involved in S-adenosylmethionine binding in the EcoRI DNA methylase. Photoaffinity laveling with 8-azido-S-adenosylmethionine.
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منابع مشابه
Identification of peptides involved in S-adenosylmethionine binding in the EcoRI DNA methylase. Photoaffinity laveling with 8-azido-S-adenosylmethionine.
The Mr 38,050 monomeric EcoRI DNA methylase is part of a bacterial restriction-modification system. The methylase transfers the methyl group from S-adenosylmethionine (AdoMet) to the second adenine in the double-stranded DNA sequence 5'-GAATTC-3'. We have used the radiolabeled photoaffinity analog 8-azido-S-adenosylmethionine (8-N3-AdoMet) to identify peptides at the AdoMet binding site in the ...
متن کاملIdentification of a unique radical S-adenosylmethionine methylase likely involved in methanopterin biosynthesis in Methanocaldococcus jannaschii.
Methanopterin (MPT) and its analogs are coenzymes required for methanogenesis and methylotrophy in specialized microorganisms. The methyl groups at C-7 and C-9 of the pterin ring distinguish MPT from all other pterin-containing natural products. However, the enzyme(s) responsible for the addition of these methyl groups has yet to be identified. Here we demonstrate that a putative radical S-aden...
متن کاملS-Adenosylmethionine and methylation.
S-Adenosylmethionine (AdoMet or SAM) plays a pivotal role as a methyl donor in a myriad of biological and biochemical events. Although it has been claimed that AdoMet itself has therapeutic benefits, it remains to be established whether it can be taken up intact by cells. S-Adenosylhomocysteine (AdoHcy), formed after donation of the methyl group of AdoMet to a methyl acceptor, is then hydrolyze...
متن کاملThe effect of methionine and S-adenosylmethionine on S-adenosylmethionine levels in the rat brain.
OBJECTIVE S-Adenosylmethionine (SAMe) is a major methyl donor in the brain and is also an antidepressant with few reported side effects; however, SAMe is relatively expensive and unstable. Brain SAMe can be increased by giving methionine to rats, raising the possibility that methionine may be an antidepressant. We aimed to study whether SAMe and methionine, when given orally to rats, could rais...
متن کاملThe preparation of S-adenosylmethionine.
Although the enzymatic synthesis of S-adenosylmethionine was accomplished several years ago (l), there have, as yet, been few reports of experiments with this compound. The main reason for the paucity of data appears to be the difficulty of obtaining S-adenosylmethionine in adequate quantities at a reasonable expenditure of time and starting material. Cantoni (2) has prepared S-adenosylmethioni...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1990
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)38977-x